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KMID : 1098420170250020095
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 2 p.95 ~ p.101
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Callus Induction and Increase in Anti-Inflammatory Activity by Treatment of Methyl Jasmonate in Adenium obesum
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Lee Da-Young
Min Jin-Woo
Joo Kwang-Sik
Kang Hee-Cheol
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Abstract
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Background: Callus cultivation has the advantage of producing a large amount of tissue of a plant in a laboratory regardless of the environment, for extracting an active substance. In the present study, callus formation was induced in the leaves of the succulent plant Adenium obesum (Forssk.) Roem & Schult. After callus cultivation, anti-inflammatory activity tests were conducted, because leaves and stems of A. obesum have been reported to possess biological activity.
Methods and Results: In order to induce callus formation, various concentrations of plant growth factors, such as kinetin, naphtha-leneacetic acid (NAA), 6-benzyladenine (BA), and indole-3-acetic acid (IAA) were added to MS solid medium. The maximum callus proliferation was induced by mixed medium consisting of NAA (2 ㎎/ ℓ ) and BA (1 ㎎/ ℓ ). In addition, an elicitor was added to the medium under optimal conditions for initiating suspension culture. After suspension culturing, the activities of the callus extracts were compared and analyzed. The cytotoxicity and anti-inflammatory activity tests revealed that the anti-inflammatory activity of the callus extract and the content of phenolic compounds were elevated after treatment of the callus culture with the elicitior.
Conclusions: A. obesum callus might be considered as potential source of biologically active anti-inflammatory material.
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KEYWORD
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Adenium obesum (Forssk.) Roem & Schult, Anti-Inflammatory Activity, Callus Induction, Elicitation, Elicitor, Polyphenol, Suspension Culture
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